Rasmussen, L., & Olapade, O. A. (2016). Influence of zinc on bacterial populations and their proteolytic enzyme activities in freshwater environments: a cross-site comparison. Canadian Journal of Microbiology, 1-9.
Abstract: Temporal responses of indigenous bacterial populations and proteolytic enzyme (i.e., aminopeptidase) activities in the bacterioplankton assemblages from 3 separate freshwater environments were examined after exposure to various zinc (Zn) concentrations under controlled microcosm conditions. Zn concentrations (ranging from 0 to 10 μmol/L) were added to water samples collected from the Kalamazoo River, Rice Creek, and Huron River and examined for bacterial abundance and aminopeptidase activities at various time intervals over a 48 h incubation period in the dark. The results showed that the Zn concentrations did not significantly influence total bacterial counts directly; however, aminopeptidase activities varied significantly to increasing zinc treatments over time. Also, analysis of variance and linear regression analyses revealed significant positive relationships between bacterial numbers and their hydrolytic enzyme activities, suggesting that both probably co-vary with increasing Zn concentrations in aquatic systems. The results from this study serve as additional evidence of the ecological role of Zn as an extracellular peptidase cofactor on the dynamics of bacterial assemblages in aquatic environments.
Horch, E. P., van Belle, G. T., Davidson, J. W., Jr., Ciastko, L. A., Everett, M. E., & Bjorkman, K. S. (2015). Observations of Binary Stars with the Differential Speckle Survey Instrument. VI. Measures During 2014 at the Discovery Channel Telescope. arXiv:1509.03498.
Abstract: We present the results of 938 speckle measures of double stars and suspected double stars drawn mainly from the Hipparcos Catalogue, as well as 208 observations where no companion was noted. One hundred fourteen pairs have been resolved for the first time. The data were obtained during four observing runs in 2014 using the Differential Speckle Survey Instrument (DSSI) at Lowell Observatory’s Discovery Channel Telescope. The measurement precision obtained when comparing to ephemeris positions of binaries with very well-known orbits is generally less than 2 mas in separation and 0.5 degrees in position angle. Differential photometry is found to have internal precision of approximately 0.1 magnitudes and to be in very good agreement with Hipparcos measures in cases where the comparison is most relevant. We also estimate the detection limit in the cases where no companion was found. Visual orbital elements are derived for 6 systems.
Erhard, K. F., Talbot, J., Deans, N. C., McClish, A. E., & Hollick, J. B. (2015). Nascent Transcription Affected by RNA Polymerase IV in Zea mays. Genetics, 199(4), 1107-U1355.
Abstract: All eukaryotes use three DNA-dependent RNA polymerases (RNAPs) to create cellular RNAs from DNA templates. Plants have additional RNAPs related to Pol II, but their evolutionary role(s) remain largely unknown. Zea mays (maize) RNA polymerase D1 (RPD1), the largest subunit of RNA polymerase IV (Pol IV), is required for normal plant development, paramutation, transcriptional repression of certain transposable elements (TEs), and transcriptional regulation of specific alleles. Here, we define the nascent transcriptomes of rpd1 mutant and wild-type (WT) seedlings using global run-on sequencing (GRO-seq) to identify the broader targets of RPD1-based regulation. Comparisons of WT and rpd1 mutant GRO-seq profiles indicate that Pol IV globally affects transcription at both transcriptional start sites and immediately downstream of polyadenylation addition sites. We found no evidence of divergent transcription from gene promoters as seen in mammalian GRO-seq profiles. Statistical comparisons identify genes and TEs whose transcription is affected by RPD1. Most examples of significant increases in genic antisense transcription appear to be initiated by 3′-proximal long terminal repeat retrotransposons. These results indicate that maize Pol IV specifies Pol II-based transcriptional regulation for specific regions of the maize genome including genes having developmental significance.
Sovansky, E. E., Wieth, M. B., Francis, A. P., & McIlhagga, S. D. (2014). Not all musicians are creative: Creativity requires more than simply playing music. Psychology of Music.
Abstract: Musical training has been found to be associated with increased creativity. However, it is not clear whether increased creativity, particularly divergent thinking, is associated with music expertise due to knowledge and skill, or if increased creativity arises from participation in the creation of music through practices such as improvisation and composition. This study investigated how level of music expertise and engagement in the creation of music relate to divergent thinking in musically trained adults (musicians). Sixty participants of varying music expertise were tested for divergent thinking using a modified version of Guilford’s (1967) alternative uses task, in which participants listed creative uses for two music items and two non-music items. Results indicate that musicians who create music listed more creative uses for music items than non-musicians and musicians who do not create music. For non-music items, participants did not display differences in divergent thinking.
Hooks, J., & Erdman, S. (2014). Turnover And Closed-End Fund Discounts. Journal of Business & Economics Research, 12(4), 335-338.
Abstract: This paper seeks to further investigate the quandary of closed-end fund discounts known as the “four-piece puzzle.” While other researchers have taken a behavior approach (investor sentiment, etc.), this study will explore empirical data on several variables, including some Fama-French factors. Using a fixed effects model, the effects of turnover, three-year-beta, price/book, median market capitalization, expenses and income were measured in this study.
Hill, E., Terrell, H., Arellano, A., Schuetz, B., & Nagoshi, C. (2014). A Good Story: Using Future Life Narratives to Predict Present Well-Being. Journal of Happiness Studies, 1-20.
Abstract: The present research examined the predictive and nomological validity of a narrative method for assessing goals. College students (N = 337, 158 women, M age = 19.08) from a large, public university wrote short narratives about their best possible selves in the future, imagining that they had realized all of their life dreams. Narratives were coded in terms of the number of statements reflecting each of fourteen types of goals. Intercoder reliability was strong. With regard to predictive validity, intrinsic goals, particularly spiritual and intimacy goals were positively related to well-being. Extrinsic goals, power goals in particular, tended to be negatively related to well-being. With regard to nomological validity, the spiritual goals-well-being relationship was mediated by frequency of religious service attendance and self-report measures of religiosity. Interestingly, intrinsic goals were negatively related to life satisfaction. Results are discussed in the context of self-determination theory and the internalization of extrinsic motivations.
Wickre, B., Martin, J., & McCauley, A. (Eds.). (2014). Images of Women in the Albion College Print Collection. Albion, MI: Celandine Press/Albion College Department of Art and Art History.
Abstract: Images of women in prints from the 15th century to the present in the Albion College Print Collection. Contributions from students and faculty.
Olapade, O. A., & Ronk, A. J. (2014). Isolation, Characterization and Community Diversity of Indigenous Putative Toluene-Degrading Bacterial Populations with Catechol-2,3-Dioxygenase Genes in Contaminated Soils. Microbial Ecology, 1-7.
Abstract: Indigenous bacterial assemblages with putative hydrocarbon-degrading capabilities were isolated, characterized and screened for the presence of the catechol-2,3-dioxygenase (C23O) gene after exposure to toluene in two different (i.e., pristine and conditioned) soil communities. The indigenous bacterial populations were exposed to the hydrocarbon substrate by the addition of toluene concentrations, ranging from 0.5 % to 10 % V/W in 10 g of each soil and incubated at 30 °C for upwards of 12 days. In total, 25 isolates (11 in pristine soil and 14 in conditioned soil) were phenotypically characterized according to standard microbiological methods and also screened for the 238-bp C23O gene fragment. Additionally, 16S rRNA analysis of the isolates identified some of them as belonging to the genera Bacillus, Exiguobacterium, Enterobacter, Pseudomonas and Stenotrophomonas. Furthermore, the two clone libraries that were constructed from these toluene-contaminated soils also revealed somewhat disparate phylotypes (i.e., 70 % Actinobacteria and Firmicutes to 30 % Proteobacteria in conditioned soil, whereas in pristine soil: 66 % Actinobacteria and Firmicutes; 21 % Proteobacteria and 13 % Bacteroidetes). The differences observed in bacterial phylotypes between these two soil communities may probably be associated with previous exposure to hydrocarbon sources by indigenous populations in the conditioned soil as compared to the pristine soil.
Wilson, W. J., Ferrara, N. C., Blaker, A. L., & Giddings, C. E. (2014). Escape and avoidance learning in the earthworm Eisenia hortensis. PeerJ, 2, e250.
Abstract: Interest in instrumental learning in earthworms dates back to 1912 when Yerkes concluded that they can learn a spatial discrimination in a T-maze. Rosenkoetter and Boice determined in the 1970s that the “learning” that Yerkes observed was probably chemotaxis and not learning at all. We examined a different form of instrumental learning: the ability to learn both to escape and to avoid an aversive stimulus. Freely moving “master” worms could turn off an aversive white light by increasing their movement; the behavior of yoked controls had no effect on the light. We demonstrate that in as few as 12 trials the behavior of the master worms comes under the control of this contingency.
McCaffrey, V. P., Zellner, N. E. B., Waun, C. M., Bennett, E. R., & Earl, E. K. (2014). Reactivity and Survivability of Glycolaldehyde in Simulated Meteorite Impact Experiments. Origins of Life and Evolution of Biospheres, 1-14.
Abstract: Sugars of extraterrestrial origin have been observed in the interstellar medium (ISM), in at least one comet spectrum, and in several carbonaceous chondritic meteorites that have been recovered from the surface of the Earth. The origins of these sugars within the meteorites have been debated. To explore the possibility that sugars could be generated during shock events, this paper reports on the results of the first laboratory impact experiments wherein glycolaldehyde, found in the ISM, as well as glycolaldehyde mixed with montmorillonite clay, have been subjected to reverberated shocks from ~5 to >25 GPa. New biologically relevant molecules, including threose, erythrose and ethylene glycol, were identified in the resulting samples. These results show that sugar molecules can not only survive but also become more complex during impact delivery to planetary bodies.
Metz, K. M., Sanders, S. E., Miller, A. K., & French, K. R. (2014). Uptake and Impact of Silver Nanoparticles on Brassica rapa: An Environmental Nanoscience Laboratory Sequence for a Nonmajors Course. Journal of Chemical Education, 91(2), 264-268.
Abstract: Nanoscience is one of the fast growing fields in science and engineering. Curricular materials ranging from laboratory experiments to entire courses have been developed for undergraduate science majors. However, little material has been developed for the nonmajor students. Here we present a semester-long laboratory sequence developed for a nonmajors course, where students investigate the potential environmental impacts of nanoscience. Students synthesize and characterize silver nanoparticles using green synthetic methods. They then use the suspension of silver nanoparticles to “water” Wisconsin Fast Plants, Brassica rapa, over a three to four week period to simulate environmental exposure. Possible impacts are examined throughout the growth period, and silver uptake by the plants is quantified at the end of the growth period. This lab requires design input from the student, making it an open-ended experiment. Although designed for nonmajors, this lab could easily be adapted for an environmental chemistry or chemical nanoscience course.
Walker, R. J., Kribs, Z. D., Christopher, A. N., Shewach, O. R., & Wieth, M. B. (2014). Age, the Big Five, and time-of-day preference: A mediational model. Personality and Individual Differences, 56, 170-174.
Abstract: This research examined the extent to which the Big Five personality factors mediated the relationship between age and time-of-day preference. A sample of 491 Americans (M-age = 32 yrs) completed the 240-item NEO-PI-R, the 19-item Home and Ostberg’s (1976) Morningness-Eveningness Questionnaire (MEQ), and provided demographic information. As demonstrated in previous research, correlations revealed that older people expressed a stronger morning preference. More importantly, using bootstrapping procedures, it was found that the Big Five factor of conscientiousness attenuated the relationship between age and time-of-day preference. These findings indicate that conscientiousness plays a significant role in the relationship between age and time-of-day preference.
Brandt, A. E., Sztykiel, H., & Pietras, C. J. (2013). Laboratory Simulated Gambling: Risk Varies Across Participant-Stake Procedure. Journal of General Psychology, 140(2), 130-143.
Abstract: This study investigated whether risk taking on a laboratory gambling task differed depending on whether participants gambled with earned or experimenter-provided game credits. Participants made repeated choices between two options, one to wager game credits on a game that produced probabilistic gains and losses, and one to gain game credits with certainty. Choice was investigated across stake and no-stake conditions and condition order was counterbalanced across conditions. Risk taking was higher under stake than no-stake conditions, but only when stake conditions were experienced first. There was no effect on risk taking of the amount of the certain gain. Results are consistent with previous research showing that participant-stake procedures promote greater risk taking than procedures that allow participants to gamble with their own earnings, and also show that experience gambling with earned credits has an enduring effect on risk taking.
Duffy, P., Reynolds, L. A., Sanders, S. E., Metz, K. M., & Colavita, P. E. (2013). Natural reducing agents for electroless nanoparticle deposition: Mild synthesis of metal/carbon nanostructured microspheres. Materials Chemistry and Physics, 140(1), 343-349.
Abstract: Composite materials are of interest because they can potentially combine the properties of their respective components in a manner that is useful for specific applications. Here, we report on the use of coffee as a low-cost, green reductant for the room temperature formation of catalytically active, supported metal nanoparticles. Specifically, we have leveraged the reduction potential of coffee in order to grow Pd and Ag nanoparticles at the surface of porous carbon microspheres synthesized via ultraspray pyrolysis. The metal nanoparticle-on-carbon microsphere composites were characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and thermal gravimetric analysis (TGA). To demonstrate the catalytic activity of Pd/C and Ag/C materials, Suzuki coupling reactions and nitroaromatic reduction reactions were employed, respectively.
Olapade, O., & Pung, K. (2012). Plant-associated bacterial populations on native and invasive plant species: comparisons between 2 freshwater environments. Canadian Journal of Microbiology, 58(6), 767-775.
Abstract: Plant–microbial interactions have been well studied because of the ecological importance of such relationships in aquatic systems. However, general knowledge regarding the composition of these biofilm communities is still evolving, partly as a result of several confounding factors that are attributable to plant host properties and to hydrodynamic conditions in aquatic environments. In this study, the occurrences of various bacterial phylogenetic taxa on 2 native plants, i.e., mayapple (Podophyllum peltatum L.) and cow parsnip (Heracleum maximum Bartram), and on an invasive species, i.e., garlic mustard (Alliaria petiolata (M. Bieb.) Cavara & Grande), were quantitatively examined using nucleic acid staining and fluorescence in situ hybridization. The plants were incubated in triplicates for about a week within the Kalamazoo River and Pierce Cedar Creek as well as in microcosms. The bacterial groups targeted for enumeration are known to globally occur in relatively high abundance and are also ubiquitously distributed in freshwater environments. Fluorescence in situ hybridization analyses of the bacterioplankton assemblages revealed that the majority of bacterial cells that hybridized with the different probes were similar between the 2 sites. In contrast, the plant-associated populations while similar on the 3 plants incubated in Kalamazoo River, their representations were highest on the 2 native plants relative to the invasive species in Pierce Cedar Creek. Overall, our results further suggested that epiphytic bacterial assemblages are probably under the influences of and probably subsequently respond to multiple variables and conditions in aquatic milieus.
Nwe, Soe Yu (“Joy”). “House.” Michigan Ceramic Art Association Michigan Mud Conference Award Winner: Michigan Ceramic Art Association, 2011.
Description: Junior Soe Yu Nwe (“Joy” on campus) took one of the top four student awards at the biennial Michigan Ceramic Art Association Michigan Mud conference held in October 2011.
Soe Yu Nwe’s winning sculpture, “House,” was inspired by a childhood spent on both sides of the border between Thailand and the Union of Myanmar. Many Thai families have “spirit houses,” small structures which are decorated and sometimes furnished for spirits tied to that land. Click on the image below for more information.
'House,' Soe Yu Nwe's winning entry
Burkholder, D. A., Heithaus, M. R., Thomson, J. A., & Fourqurean, J. W. (2011). Diversity in trophic interactions of green sea turtles Chelonia mydas on a relatively pristine coastal foraging ground. Marine Ecology-Progress Series, 439, 277-293.
Abstract: Adult green sea turtles Chelonia mydas are often the largest-bodied herbivores in their communities and may play an important role in structuring seagrass and macroalgal communities. Recent studies, however, suggest that green turtles might be more omnivorous than previously thought. We used animal-borne video and nitrogen and carbon stable isotopic analysis of skin to elucidate diets of green turtles in the relatively pristine seagrass ecosystem of Shark Bay, Australia. Stable isotope values suggested that despite the presence of abundant seagrass resources, turtles assimilated most of their energy from a combination of macroalgae and gelatinous macroplankton (cnidarians and ctenophores). Video data suggested that macroplankton might be the most commonly consumed food source. Also surprising was the considerable variation in delta(13)C values, suggesting long-term dietary specialization by individual turtles. Overall, green turtle foraging under natural conditions may be less stereotyped than previously thought, and diets of green turtles inhabiting apparently similar ecosystems (e. g. seagrass-dominated ecosystems) may vary considerably across geographical regions. The apparently high degree of individual specialization in diets suggests that conservation efforts should account not only for the potential importance of non-benthic food sources for green turtle populations, but also for the possibility that subsets of the population may play different ecological roles and may be differentially vulnerable to anthropogenic impacts.
Edwards, S. (2012). Good Grief. Long Beach, CA: Write Bloody Publishing.
Publisher’s Description: Stevie Edwards’ debut book of poetry, Good Grief, catalogues her elegantly-wrought misadventures as a freshly-graduated, Michigan transplant stumbling over foal legs through Chicago and kneeling down to confront the wreckage of her skinned knees. Whether stopping to disinter some small ruin of a secondhand-clothes childhood, charting the reaches of her own privilege as a white woman in Chicago, or trying to recollect the reasoning behind last night’s bar receipts, Stevie’s voice — a treble, equal parts angst and grace — rumbles deep down in the belly of her poems, and lingers.
Bindman, N., Merkx, R., Koehler, R., Herrman, N., & van der Donk, W. A. (2010). Photochemical cleavage of leader peptides. Chemical Communications, 46(47), 8935-8937.
Abstract: We report a photolabile linker compatible with Fmoc solid phase peptide synthesis and Cu(I)-catalyzed alkyne-azide cycloaddition that allows photochemical cleavage to afford a C-terminal peptide fragment with a native amino terminus. (Journal abstract)
Olapade, O. A., Pung, K., Goldman, E., & Lyons-Sobaski, S. (2011). Occurrence and diversity of epiphytic bacterial communities on two native plant species in a Michigan Creek. Journal of Freshwater Ecology, 26(2), 267 – 276.
Abstract: We examined the occurrence and diversity of bacteria of different phylogenetic groups in epiphytic assemblages on two native Michigan plant species mayapple (Podophyllum peltatum) and cow parsnip (Heracleum maximum) when the plant leaves were submerged in fresh water. Fresh leaves were incubated in triplicate for about a week within a creek in Hasting, Michigan, and in laboratory microcosm to develop mature epiphytic assemblages. We enumerated bacteria in these assemblages by nucleic acid staining (i.e., total direct counts using 4′,6-diamidino-2-phenyllindole) and fluorescent in situ hybridization (FISH), while community diversity was determined based on 16S rRNA gene sequencing. FISH indicated the dominance of members of the γ–Proteobacterial subclass (20%) on both plant species, while the 16S rRNA gene analysis revealed the predominance of the β–Proteobacteria (51%) on mayapple and the Fermicutes (26%) on cow parsnip, with the Bacteroidetes present equally within the epiphytic assemblages on both plants.
Togunde, D., & Rinkinen, J. (2010). Homogeneous Faith, Ethnic Diversity: Desirable and Undesirable Traits in a Marital Partner in Nigeria. International Journal of Diversity in Organizations, Communities and Nations, 10(1), 219-238.
Abstract: This paper draws on survey data gathered in 2007 from over 2000 students in six Nigerian universities to investigate desirable and undesirable traits in a future marital partner and how these traits vary by gender. Until now, there is no single study in the African context that examines how measures of Westernization and globalization impact qualities desired in a mate. Findings indicate that a vast majority of respondents prefer to select their future marital partner on their own rather than through an arranged marriage. Yet an overwhelming proportion of students are unwilling to marry someone without the consent of their parents. Respondents would prefer not to marry a partner who: does not possess a comparable university education; does not want to have children; lack domestic skills; are not good at cooking; does not believe in God; and practices a different religion. However, respondents are more willing to marry someone who: comes from different tribal/ethnic group or nationality; and has had previous sexual relations. Significant gender differences were found to exist in traits such as domestic skills, age difference between spouses, level of education, parental socio-economic status, and desire to have children. The conclusion is that a simultaneous operation of traditional and contemporary mating dynamics is taking place in Nigeria. The urban-based respondents seem to hold on to some aspects of African traditional culture and practices regarding desirable and undesirable traits in a marital partner. At the same time, the criteria for mate selection are being impacted by forces of Westernization and globalization, such as the internet and foreign mass media.
Togunde, D., Osagie, S., & Rinkinen, J. (2010). Dating Patterns and Practices in the Era of Globalization in Nigeria. Global Studies Journal, 3(2), 67-84.
Abstract: This paper seeks to understand dating patterns and practices among Nigerian undergraduate students in the era of globalization. Drawing on data collected in 2007 from over 2,000 students in six universities, the paper investigates the patterns, avenues, and motivations for dating; explores the onset of dating and determines whether or not respondents have ever dated or are currently dating a person of the same sex; and whether they utilize the Internet, newspapers/magazines, and television programs to find their romantic partners. Results reveal that both the classrooms and religious places of worship (churches and mosques) are the dominant avenues for finding mates. An overwhelming proportion (70.5%) indicated that their most important reason for dating is to find a future marital partner, followed by the desire to experience love and companionship (22.7%). Number of partners dated at a time, use of the Internet, newspapers/magazines and television as avenues for meeting partners vary significantly by respondents’ gender, religion, and place of birth. However, an overwhelming number of both males and females believe that males should pay for dating expenses although a lower proportion does so in practice. An insignificant percentage of respondents admitted to having same-sex relationships. Overall, the conclusion is that a cultural dualism exists as Western dating culture co-exists with traditional dating practices. The study provides an opportunity to uncover the extent to which modernization and globalization affect intimate relationships such as dating in a transitional society.
Tingley, R., Herman, T., Pulsifer, M., McCurdy, D., & Stephens, J. (2010). Intra-Specific Niche Partitioning Obscures the Importance of Fine-Scale Habitat Data in Species Distribution Models. Biodiversity and Conservation, 19(9), 2455-2467.
Abstract: Geographic information systems (GIS) allow researchers to make cost-effective, spatially explicit predictions of species’ distributions across broad geographic areas. However, there has been little research on whether using fine-scale habitat data collected in the field could produce more robust models of species’ distributions. Here we used radio-telemetry data collected on a declining species, the North American wood turtle (Glyptemys insculpta), to test whether fine-scale habitat variables were better predictors of occurrence than land-cover and topography variables measured in a GIS. Patterns of male and female occurrence were similar in the spring; however, females used a much wider array of land-cover types and topographic positions in the summer and early fall, making it difficult for GIS-based models to accurately predict female occurrence at this time of year. Males on the other hand consistently selected flat, low-elevation, riparian areas throughout the year, and this consistency in turn led to the development of a strong GIS-based model. These results demonstrate the importance of taking a more sex-specific and temporally dynamic view of the environmental niche.
Zabel, K. L., Christopher, A. N., Marek, P., Wieth, M. B., & Carlson, J. J. (2009). Mediational Effects of Sensation Seeking on the Age and Financial Risk-Taking Relationship. Personality and Individual Differences, 47(8), 917-921.
Abstract: The current study examined the potential mediating role of sensation seeking in the well-established negative relationship between age and financial risk-taking. A total of 299 participants, aged 17-90years, allocated hypothetical money into mutual funds that varied in risk and completed a sensation seeking measure. Hierarchical multiple regressions revealed that the amount of variability age accounted for in risk-taking (4.1%; [beta] =-.22) was significantly reduced when sensation seeking was controlled for (0.8%; [beta] =-.12). A Sobel test revealed that sensation seeking fully mediated the aforementioned relationship. Results suggest sensation seeking’s role as a mediator in more physiologically arousing risk-taking contexts (e.g., surfing). Discussion recommends investigating potential biologically and cognitively-rooted mediators and moderators of the age and risk-taking relationship.
Yoo, G. H., Kafri, Z., Ensley, J. F., Lonardo, F., Kim, H., Folbe, A. J., Won, J., Stevens, T., Lin, H. (2010). Xrp6258-Induced Gene Expression Patterns in Head and Neck Cancer Carcinoma. The Laryngoscope. Published online April 20, 2010.
Abstract: XRP6258 is a novel taxoid, which has antitumor activity in preclinical mouse orthotopic and human xenograft cancer models. However, limited XRP6258 studies have been performed in head and neck squamous cell carcinoma cells (HNSCC). The objective of this study is to identify the antitumor activity of XRP6258 in HNSCC cell line models.HNSCC cells (HN30 and HN12) were exposed to either XRP6258 or docetaxel. XRP6258-induced growth suppression, cell cycle arrest and apoptosis were measured. Further, XRP6258-induced expression patterns of selected genes were compared to docetaxel-induced expression patterns using Western blot analysis.XRP6258 suppressed proliferation and induced G2M arrest and apoptosis in both of the cell lines tested. XRP6258 and docetaxel produced similar alteration in the expression of cell cycle regulators, such as cyclin A and cyclin B1. The expression of E2F and EGFR were decreased in both XRP6258 and docetaxel-treated HNSCC cells. Finally, XRP6258 induced a greater level of bcl2 phosphorylation than docetaxel in HN12 cell line.XRP6258 appeared to have a similar mechanism of action as docetaxel in the two HNSCC cell lines studied. XRP6258 induced cell cycle arrest, growth suppression, and apoptosis by altering gene expression patterns similar to that induced by docetaxel. These preclinical experiments suggest that XRP6258 may be useful in treating HNSCC, and the aforementioned genes can potentially be used as surrogate endpoint biomarkers. Laryngoscope, 2010
Flannery, E., VanZomeren-Dohm, A., Beach, P., Simanton Holland, W., & Duman-Scheel, M. (2010). Induction of Cellular Growth by the Axon Guidance Regulators Netrin a and Semaphorin-1a. Developmental Neurobiology, 70(7), 473-484.
Abstract: Although neurite outgrowth has been linked to axon guidance regulators, the effects of guidance molecules on cellular growth are not well understood. Use of the Drosophila wing imaginal disc, an epithelial tissue and a well-characterized system for analysis of cellular growth regulation, permits analysis of the impacts of guidance molecules on cellular growth in a setting in which axon guidance is not a confounding factor. In this investigation, the impacts of Netrin A (NetA) and Semaphorin-1a (Sema1a) signaling on cellular growth are examined during wing development. Levels of these genes were modulated in somatic clones in the developing wing disc, and clone areas, as well as individual sizes of clonal cells were assessed. NetA and Sema1a signaling were found to induce cellular growth in these assays. Furthermore, immunohistochemical analyses indicated that NetA and Sema1a signaling induce expression of several growth regulators, including myc, cycD, cdk4, PCNA, and MapK in the wing disc. These data illustrate that NetA and Sema1a can specifically promote growth through induction of key cellular growth regulators. The abilities of NetA and Sema1a to regulate cellular growth are likely critical to their functions in both nervous system development and oncogenesis. © 2010 Wiley Periodicals, Inc.
Olapade, O. A., & Weage, E. A. (2010). Comparison of Fecal Indicator Bacterial Populations in Surface Waters of the Kalamazoo River, USA. Microbes and Environments, 25(1).
Abstract: Surface waters along the Kalamazoo River, USA, were examined for occurrence and population trends of fecal indicator bacteria (FIB) with culture-based and culture-independent methods. The two methods recorded discrepancies in FIB counts, with the culture-independent method revealing more consistent numbers between the river sites. FIB cells that hybridized with the ECO1482 probe were highest in the downstream site, while the upstream site recorded higher ENF343 hybridized cells. Spatial and temporal differences in FIB populations were probably attributable to contrasting fecal pollution influences, vegetation type, varying environmental conditions as well as several in-stream factors between the two river sites.
Guenin-Lelle, D., & Harris, A. (2009). The Role of Music Festivals in the Cultural Renaissance of Southwest Louisiana in the Late Twentieth Century. Louisiana History, 50(4), 461-472.
Togunde, D., & Rinkinen, J. (2009). Agents of Change: Gender Differences in Migration Intentions among University Undergraduates in Nigeria. International Journal of Interdisciplinary Social Sciences, 4(2), 175-190.
Abstract: This paper draws on surveys/interviews with 678 Nigerian university undergraduates to examine migration intentions and to detect if gender differences exist in reasons to migrate (or not) to the United States. This study is unique by focusing on future migration among university students, whose views and migration plans have been neglected in previous studies. As a departure from few previous scholarships in Africa, the paper introduces two new variables: perception of America as a land of socio-economic opportunities and whether respondents actively participate in the U.S. Visa Lottery Program. Findings indicate that a higher proportion of males than females cites better employment opportunities as reason for planning to move. However, more females than males mention security and better infrastructures available in America as motives for wanting to emigrate within the next five years. A higher proportion of women than men mention social and cultural ties with homeland and perception of racism in America as factors discouraging them from wanting to live in the United States; whereas, more men than women wanting to stay in Nigeria refer to patriotism/love of homeland as reasons. Perception of America as a land of opportunities and active participation in the U.S. Visa Lottery Program are among significant predictors of intentions to migrate. Findings have implications for policies aimed at improving quality of life in Nigeria, thereby, reducing emigration of “future leaders of tomorrow”.
Perkins, E., Stephens, J., Xiang, H., & Lo, W. (2009). The Cost of Pediatric Stroke Acute Care in the United States. Stroke, 40(8), 2820-2827.
Abstract: Background and Purpose–The cost of pediatric stroke care has received little attention, but the available data suggest it is expensive. To determine the cost of acute stroke, we analyzed a US national database.Method–We used the Kids’ Inpatient Database (KID2003) to determine the hospital-based costs of acute stroke in children ages 3 months to 20 years. Discharges were selected if the first diagnostic position contained an International Classification of Diseases, 9th Revision code pertaining to ischemic or hemorrhagic stroke. We examined the relationship between cost and stroke type by adjusting for variables that predict the cost of adult stroke.Results–There were 2224 pediatric cases, after statistical weighting, discharged with a diagnosis of hemorrhagic or ischemic stroke in KID2003. The estimated cost of acute pediatric stroke in the United States was $42 million in 2003. For the entire cohort, the mean cost of acute hospital care was $20 927 per discharge. The mean cost for ischemic stroke was $15 003, for intracerebral hemorrhage $24 117, and for subarachnoid hemorrhage $31 653. Stroke diagnosis, length of stay, hospital ownership, rural/urban teaching status, US geographical region, and discharge disposition were significantly associated with cost. Cost remained significantly associated with stroke diagnosis after adjusting for other predictors in the final multivariable regression model.Conclusions–Pediatric stroke is expensive, and the lifetime cost of care is likely greater for a child than an adult. The cost to the family and the larger society underscore the importance of pediatric stroke treatment and prevention.